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Food Safety
Applications
Pesticide Analysis in Wine
Using QuEChERS Methodology
(Part Number: 60105-205 and 60105-211)
Sample Preparation
Add 20mL ACN and internal standard fluconazole (250µL)
to 60105-211 Quantitatively add 20.0mL of wine
Shake for approximately 2 minutes
Centrifuge at 4500rpm for 5 minutes (use refrigerated
centrifuge if available)
Transfer 9.0mL of top layer and add to 60105-205
Mix/vortex tube for approximately 10 seconds
Open tube and add 3.0mL of toluene and shake for
1 minute
Centrifuge the tube for 5 minutes at 4500rpm
Quantitatively transfer 2.0mL of supernatant to a glass
centrifuge tube
Evaporate to dryness at <40˚C using N
2
Add 500µL of ACN 25µL of benzanilide (2.0µg/L)
surrogate standard for QC and 500µL of 20mM
ammonium acetate in 1% ACN to the dried extract
Mix/vortex for approximately 5 seconds and filter into
autosampler vial using 17mm, 0.2µm nylon membrane
syringe filter (F2513-2) attached to a disposable syringe
Analysis
Inject 3µL onto appropriate LC/MS system
Monitor the following transitions:
Compound
MRM Transition
Acephate
Acetamiprid
Acibenzolar S-methyl
Aldicarb
Aldicarb sulfone
Aldicarb sulfoxide
Atrazine
Avermectin B 1b
Avermectin B1a
Azoxystrobin
Benalaxyl
Benfuracarb
Benzanilide
Bifenazate
Bitertanol
Buprofezin
Carbaryl
Carbendazim
Carbofuran
Chloroxuron
Cyprodinil
Cyromazine
Diclobutrazol
Dimethoate
Dimethomorph
Dimoxystrobin
Dinotefuran
Diuron
Compound
MRM Transition
Ethofumesate
Famoxadone
Fenamidone
Fenbuconazole
Fenhexamid
Fenpropimorph
Fluconazole
Fludioxinil
Furathiocarb
Hexaconazole
Imazalil
Imidacloprid
Ipconazole
Iprovalicarb
Kresoxim-methyl
Mepanipyrim
Metalaxyl
Methamidophos
Methomyl
Methoxyfenozide
Mevinphos
Myclobutanil
Omethoate
Oxadixyl
Piperonyl butoxide
Prochloraz
Propamocarb
Propargite
Propiconazole
Propoxur
Pyraclostrobin
Pyridaben
Pyrimethanil
Quinoxyfen
Rotenone
Simazine
Spinosyn A
Spinosyn D
Spiroxamine
Tebuconazole
Thiabendazole
Triadmimefon
Trifloxystrobin
Triflumizole
Vamidothion
Zoxamide
Recommended HPLC Column
Part Number
Hypersil GOLD 3µm, 150 x 2.1mm
25003-152130
184.0/143.0
223.4/126.1
211.1/136.0
208.1/116.0
240.0/222.9
224.2/206.9
215.9/173.85
876.6/553.4
890.7/567.5
404.0/372.1
326.1/148.1
411.2/190.0
198.1/105.1
301.3/170.2
338.2/99.1
306.3/201.2
202.1/145.1
192.0/160.0
222.1/123.1
291.0/72.2
226.1/93.0
167.2/85.1
328.1/70.2
230.1/199.0
388.0/301.1
327.1/206
203.5/14.0
233.0/72.1
286.9/258.9
373.2/282
312.2/236.2
337.1/125.0
301.9/261.9
304.4/147.1
307.2/220
247.0/180.0
383.2/195.1
314.0/70.2
297.1/159.0
256.1/175.0
334.1/70.2
321.2/119.0
314.1/116.0
224.4/77.3
280.1/220.1
142.0/94.0
163.0/88.0
369.5/149.0
225.1/192.8
289.1/70.2
214.1/183.0
279.1/219.1
356.2/177.0
376.1/308.0
189.1/102.1
368.1/231.0
342.0/159.0
210.0/111.0
388.0/194.0
365.3/309.1
200.1/107.0
307.8/196.8
395.3/213.2
202.2/131.4
732.6/142.2
746.6/142.2
298.2/144.0
308.2/70.2
202.0/175.0
294.0/197.1
409.0/186.0
346.0/278.1
288.1/146.0
336.0/187.0