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Food Safety
Applications
Trichothecene Analysis (A and B) In Wheat and Corn
Using QuEChERS Methodology
(Part Number: 60105-211 and 60105-219)
Sample Preparation
Thoroughly homogenize a sample of grain products using
a laboratory mill
Weigh 5g of sample into the 50mL centrifuge tube
Add 10mL of
methanol:ACN(85:15) into 50mL
centrifuge tube
Shake to disperse solvent
Add the contents of the 60105-211 pouch containing
4g anhydrous magnesium sulfate, 1g sodium chloride
to the centrifuge tube
Mix/vortex for 1 minute then centrifuge at 4,000rpm for
10 minutes
Sample Cleanup
Transfer a 1mL aliquot to a 2mL 60105-219 tube
(150mg anhydrous magnesium sulfate and 50mg PSA)
Shake for 1 minute
Centrifuge for 10 minutes at 4,000rpm
Filter extract through a 0.45µm filter into an LC injection
vial if supernatant is not clear
Sample is now ready for analysis
Analysis
Mass spectrometer detection with atmospheric pressure
ionization (API) configured for electrospray positive
ion mode
Flow rate 0.5mL/minute
Mobile phase:
Time:
1% Methanol%
1% Formic Acid%
0
60
40
10
10
90
25
10
90
Mass Ions for Mycotoxins [Na+M]
Ion
m/z
NIV
355
DON
319
DAS
389
HT2
447
T2
489
Recommended HPLC Column
Part Number
Betasil C18 250mm x 4.6mm x 5µm
70105-254630