Page 59 - CRFTApplication Compendium_090613

Figure 5: Using FAIMS. Representative LC-FAIMS-MS/MS chromatogram for testosterone and the lower trace is for the internal standard.
Note that the absolute signal from FAIMS analysis is lower than without FAIMS because of the removal of interferences.
0.0 0.5 1.0 1.5 2.0 2.5
2.74
2.75
3.29
3.26
3.23
3.0 3.5
Time (min)
0
20000
40000
60000
80000
100000
120000
140000
Intensity
0
1000
2000
3000
4000
5000
6000
7000
Intensity
Conclusions
A FAIMS-equipped TSQ Quantum Ultra™ triple quadru-
pole mass spectrometer provides more accurate results by
eliminating the chemical noise that arises from mobile
phase and sample matrix. In cases where interferences
prevent accurate and precise determination, FAIMS
removes interferences. The resulting chromatograms accu-
rately represent the concentration of testosterone in the
samples. The LLOQ was improved four-fold compared to
the LC-MS/MS method.
References
1 Simultaneous analysis of prostanoids using liquid chromatography/high-
field asymmetric waveform ion mobility spectrometry/tandem mass spec-
trometry. Kapron et al. Rapid Commun. Mass Spectrom. 2006; 20:
1504-1510.
2 Determination of steroids from serum and/or plasma using turbulent flow
chromatography. Cohesive Technologies; Application Note 9.0-0305; 2005.
Testosterone-d 3 internal
standard with FAIMS
Testosterone
with FAIMS