References
1. Kapron, J.; Jemal, M.; Duncan, G.; Kolakowski, B.; Purves, R. “Removal
of metabolite interference during liquid chromatography/tandem mass
spectrometry using high-field asymmetric waveform ion mobility spec-
trometry”;
Rapid Commun. Mass Spectrom.
2005
,
19
(14), 1979–1983.
2. Kapron, J.; Wu, J.; Mauriala, T.; Clark, P.; Purves, R.; Bateman, K.
“Simultaneous analysis of prostanoids using liquid chromatography/high-
field asymmetric waveform ion mobility spectrometry/tandem mass spec-
trometry”;
Rapid Commun. Mass Spectrom.
2006
,
20
(10), 1504–1510.
View additional Thermo Scientific LC/MS application notes at:
www.thermo.com/appnotes
AN62354_E 09/07S
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0.1 Da Peak Width
Y = –2108+12008.1*X–11.0775*X^2 R^2 = 0.9998 W: Equal
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Figure 6: Representative regression analysis from the LC–FAIMS–H-SRM analysis of clenbuterol
in human urine, using high resolution (0.1 FWHM) combined with FAIMS selectivity
Table 2: Comparison of QC samples from LC–SRM and LC–FAIMS–H-SRM analysis. Concentrations
exceeding bioanalytical criteria are framed. The precision and accuracy were improved to within the
guidance criteria by using FAIMS and H-SRM.
1...,336,337,338,339,340,341,342,343,344,345 347,348,349,350,351,352,353,354,355,356,...374