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Method A
Column: Hypersil GOLD aQ, 1.9 µm, 100 x 2.1 mm
A: Water, 0.1% FA
B: Acetonitrile, 0.1% FA
Flow Rate: 750 µL/min
Time (min)
Eluent B%
0.00
2.0
6.00
2.0
12.00
95.0
14.00
95.0
14.10
2.0
16.00
2.0
Method B
Column: Hypersil GOLD, 1.9 µm, 100 x 2.1 mm
A: Water, 0.1% FA
B: Acetonitrile, 0.1% FA
Flow Rate: 1000 µL/min
Time (min)
Eluent B%
0.00
1.0
6.00
1.0
12.00
95.0
14.00
95.0
14.10
1.0
16.00
1.0
Method C
Column: Hypersil GOLD PFP, 1.9 µm, 100 x 2.1 mm
A: Water, 0.1% FA
B: Acetonitrile, 0.1% FA
Flow Rate: 1000 µL/min
Time (min)
Eluent B%
0.00
0.0
6.00
0.0
10.00
30.0
14.00
60.0
14.10
0.0
16.00
0.0
Method D
Column: Hypersil GOLD PFP, 1.9 µm, 100 x 2.1mm
A: Water, 0.02% TFA
B: Acetonitrile, 0.02% TFA
Flow Rate: 1000 µL/min
Time (min)
Eluent B%
0.00
2.0
2.80
10.0
4.00
55.0
4.50
60.0
5.00
60.0
5.10
95.0
5.70
95.0
5.80
2.0
8.00
2.0
Method E
Column: Hypersil GOLD PFP, 1.9 µm, 100 x 2.1 mm
A: Water (0.06% AA)
B: Acetonitrile (0.06% AA)
Flow Rate: 1000 µL/min
Time (min)
Eluent B%
0.00
2.0
5.00
20.0
5.10
50.0
7.00
60.0
8.60
95.0
9.90
95.0
10.00
2.0
12.00
2.0
Method F
Hypersil GOLD, 1.9 µm, 50 x 2.1 mm
Hypersil GOLD PFP, 1.9 µm, 50 x 2.1 mm
A: Water (0.06% AA)
B: Acetonitrile (0.06% AA)
Flow Rate: 1000 µL/min
Time (min)
Eluent B%
0.00
2.0
5.00
20.0
5.10
50.0
7.00
60.0
8.60
95.0
10.0
95.0
10.10
2.0
12.00
2.0
Method G
Hypersil GOLD PFP, 1.9 µm, 50 x 2.1mm
Hypersil GOLD, 1.9 µm, 50 x 2.1 mm
A: Water (0.06% AA)
B: Acetonitrile (0.06% AA)
Flow Rate: 1000 µL/min
Time (min)
Eluent B%
0.00
1.5
3.00
5.0
4.50
25.0
6.00
65.0
9.50
95.0
10.0
95.0
10.10
1.5
12.00
1.5
Method H
Hypersil GOLD PFP, 1.9 µm, 100 x 2.1 mm
Hypersil GOLD, 1.9 µm, 20 x 2.1 mm
A: Water (0.06% AA)
B: Acetonitrile (0.06% AA)
Flow Rate: 1000 µL/min
Time (min)
Eluent B%
0.00
2.0
10.00
95.0
11.00
95.0
11.10
2.0
12.00
2.0
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Appendix A
AN62904_E 10/08M
Thermo Fisher Scientific,
San Jose, CA USA is ISO Certified.
1...,332,333,334,335,336,337,338,339,340,341 343,344,345,346,347,348,349,350,351,352,...374