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Figure 3: Comparison of hybrid stationary phase chemistry for the separation of 14 illicit drugs.
A) 50 x 2.1 mm Hypersil GOLD + 50 x 2.1 mm Hypersil GOLD PFP, Method F; B) 50 x 2.1 mm Hypersil
GOLD PFP + 50 x 2.1 mm Hypersil GOLD, Method G; C) 100 x 2.1 mm Hypersil GOLD PFP + 20 x 2.1 mm
Hypersil GOLD, Method H. See Appendix A for method details.
Figure 4: Optimized UHPLC/MS separation of 14 illicit drugs with ternary gradient, listed in Method I.
Conclusions
Fourteen illicit drugs and metabolites
are baseline separated in twelve minutes
by employing UHPLC/MS with a
ternary solvent gradient. Various
selectivities are achieved by different
column surface chemistry, acidic
solvent modifier and eluent system.
These results are useful for method
developments of drug identification
and quantitation. Detection by single
quadrupole MS at the ppb (ng/mL)
level is more than sufficient to
identify and quantify illicit drugs in
real samples.
References
1. C. Koeppel and J. Tenczer: Scope and limita-
tions of a general unknown screening by gas
chromatography-mass spectrometry in acute
poisoning. J. Am Soc. Mass Spectrom. 1995, 6,
995–1003.
2. W. Weinmann, A. Wiedemann, B. Eppinger, M.
Renz and M. Svoboda: Screening for drugs in
serum by electrospray ionization/collision-
induced dissociation and library searching. J.
Am Soc Mass Spectrom. 1999, 10, 1028–1037.
3. Catalog, Chromatography Columns and
Consumables, 08–09, Thermo Scientific,
page 72–82.