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Thermo Scientific Poster Note
•
PN64226-MSACL-EN 0814S
20
Relative
20
Relative
0.00
0.05
0.10
0.15
0.20
0.25
0.30
Time (min)
0
e
0.00
0.05
0.10
0.15
0.20
0.25
0.30
Time (min)
0
e
T bl 5 I t A (3 d ) P i i
(%CV 5) d A
a e . n er- ssay - ays rec s on
, n= an ccuracy
(%Diff, n=5) obtained by staggered injection across Channel 1 (CH1)
and Channel 2 (CH2) of a Multi-Channel LC.
rties used
QC L l
CH1
CH1
CH2
CH2
.
Analyte
eve
(ng/mL)
%CV
%Diff
%CV %Diff
BUP
10
8 7
7 4
9 7
6 7
.
.
.
.
800
3 3
3 3
4 2
4 4
.
.
.
.
1600
2 9
2 8
3 9
3 8
.
.
.
.
NBUP
10
9.5
7.3
10.8
7.8
800
4.7
4.8
2.7
5.1
1600
4.6
3.5
3.4
5.6
C l i
onc us ons
Use of a Transcend II UHPLC system with high-throughput multi-
,
channeling LC configured with a Q Exactive Plus Hybrid
Quadrupole-Orbitrap MS using HRAM and a Targeted-MS2
method resulted in a fast sensitive and highly specific forensic
,
,
assay in urine for BUP and NBUP.
The excellent relative recoveries of BUP and NBUP with their
i
IS i
h d
i
l
d
h
f
respect ve n t e onor ur ne samp es emonstrates t at use o
m the MS
appropriate IS can overcome matrix interferences.
ientific™
software
It is expected that the two-hour beta-glucuronidase hydrolysis as
.
bl 4 I
described herein would be sufficient for analysis of donor urine
e . on
t i i
th
d
con a n ng ese exogenous rugs.
F t
k ill f
f li i
ti
t i i t f
u ure wor w ocus on ways o e m na ng ma r x n er erences
which caused absolute analyte recoveries in donor samples to be
ion and
significantly reduced.
R f
ifi I
e erences
er on
t Mass
1 Yuan et al Quantification of Buprenorphine Norbuprenorphine
.
.,
,
and 6 Monoacet lmorphine in Urine b Liq id Chromatograph
-
y
y u
y-
Tandem Mass Spectrometry,
J Chromat Separation Techniq
2013
,
4:3
7536
.
Surine
TM
is a trademark of DYNA-TEK Industries, Lenexa, KS. All other trademarks
7536
are the property of Thermo Fisher Scientific and its subsidiaries.
Thi i f
ti
i
t i t d d t
f th
d t i
s n orma on s no n en e o encourage use o ese pro uc s n any manners
that might infringe the intellectual property rights of others.
7536