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Forensic
Applications
Dextromethorphan and Phencyclidine in Whole Blood and Urine
Using 200mg 6mL HyperSep Retain-CX Extraction Column
(Part Number: 60107-314)
Sample Preparation
1mL of 100mM phosphate buffer (pH 6)
Add internal standard*
Add 1mL blood, urine
Add 2mL of 100mM phosphate buffer (pH 6)
Mix/vortex
Centrifuge
Condition HyperSep Retain-CX Extraction Column
1 x 3mL CH
3
OH
1 x 3mL DI H
2
O
1 x 1mL 100mM phosphate buffer (pH 6)
NOTE:
Aspirate at <3"Hg to prevent sorbent drying out
Apply Sample
Load sample at 1 to 2mL/minute
Wash Column
1 x 3mL DI H
2
O
1 x 3mL 100mM acetic acid
1 x 3mL CH
3
OH
Dry column (10 minutes at >10"Hg)
Elute
1 x 3mL ethyl
acetate:ACN:ammonium hydroxide (78:20:2)
Collect eluate at 1 to 2mL/minute
Evaporate eluates under a gentle stream of nitrogen <40°
Analysis
Dissolve residue in 50µL CH
3
OH, and inject 5µL of
sample into LC/MS
Compound
MRM Transition
PCP
244.3/159.2
PCP-D5
249.3/264.1
Dextromethorphan
272.1/128.1
Dextromethorphan-D3*
275.1/131.0
* Suggested internal standard: Dextromethorphan-D3
Recommended HPLC Column
Part Number
Hypersil GOLD 3µm, 150 x 4.6mm
25003-154630