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Carisoprodol and Meprobamate in Urine, Blood, Plasma/Serum
and Tissue for GC or GC/MS Confirmations
Using 200mg 10mL HyperSep Verify-CX Extraction Column
(Part Number: 60108-742)
Prepare Sample
To 1mL of 100mM phosphate buffer (pH 3.0) add
internal standard*
Add 1mL of urine, blood, plasma/serum or 1g (1:4)
tissue homogenate
Add 2mL of 100mM phosphate buffer (pH 3.0)
Mix/vortex
Centrifuge as appropriate
Condition Verify-CX Extraction Column
3mL of CH
3
OH then aspirate
3mL of DI H
2
O then aspirate
1mL of 100mM phosphate buffer (pH 3.0) then aspirate
NOTE:
Aspirate at <3"Hg to prevent sorbent drying
Apply Sample
Load sample at 1 to 2mL/minute
Wash Column
4mL of DI H
2
O
2mL of 100mM HCl
Dry column (5 minutes at >10"Hg)
3mL of hexane
Elute Carisoprodol/Meprobamate
3mL of CH
2
Cl
2
/IPA/NH
4
OH (78:20:2)
Collect eluate at 1 to 2mL/min
NOTE:
Prepare elution solvent fresh daily; add IPA/NH
4
OH mix,
then add CH
2
Cl
2
(pH 11-12)
Dry Eluate
Evaporate to dryness at <40°C
Quantitate
Reconstitute with 100µL ethyl acetate
Inject 1 to 2µL onto gas chromatograph
For mass spectrometry, monitor the following ions:
Compound
Primary Ion** Secondary
Tertiary
Carisoprodol
158
104
245
Meprobamate
83
114
144
Hexobarbital*
221
157
81
Meprobamate-D7*
90
121
151
* Suggested internal standard
** Quantitation ion
Recommended GC Columns
Part Number
TRACE TR-DoA5 Column, 15m x 0.25mm x 0.25µm
26AF130P
TRACE TR-DoA35 Column, 15m x 0.20mm x 0.33µm
26AC497P
80
Forensic
Applications