79 / 144
79
Forensic
Applications
Carboxy THC in Urine
Using 30mg 3mL HyperSep Retain-CX Extraction Column
(Part Number: 60107-302)
Sample Preparation
2mL of urine
Add internal standard*
Add 100µL 10N NaOH
Mix/vortex
Hydrolyze for 20 mins at 60°C
Cool before proceeding
Adjust sample pH to 3.5±0.5 with 1.0mL glacial acetic acid
Apply Sample
Load at a rate of 1 to 2mL/min
Condition HyperSep Retain-CX Extraction Column
1 x 1mL DI H
2
O
1 x 1mL 0.1M HCl/ACN (70/30)
Dry column (3 mins at >10"Hg)
1 x 200µL hexane
2 x 0.5mL hexane/ethyl acetate (50:50)
Collect eluate at 1 to 2mL/min
Evaporate eluate to dryness at <40°C
Derivatize
Add 50µL ethyl acetate
Mix/vortex
Add 50µL BSTFA (1% TMCS)**
Cap
Mix/vortex
Heat for 20 minutes at 70°C
Allow to cool
NOTE:
Do not evaporate BSTFA solution
Analysis
Inject 1 to 2µL onto GC/MS
For mass spectrometry monitor the following ions:
Compound (TMS)
Target (Quantitation) Ion
Qualifier Ions
Carboxy-THC-TMS
371
473, 488
Carboxy-THC-D3-TMS*
374
476, 491
* Suggested internal standards: Carboxy-THC-D3-TMS
** Part number TS-38831
Recommended GC Column
Part Number
TraceGOLD TG-35MS 30m x 0.25m x 0.25µm
26094-1420