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Cefoperazone in Water
Using 60mg 3mL HyperSep Retain PEP Extraction Column
(Part Number: 60107-203)
Sample Preparation
5ppm of cefoperazone in water
Condition Retain PEP Extraction Column
2mL of methanol
2mL of DI H
2
O
Apply Sample
Load 10mL samples at 1-2mL/minute
Wash Column
1mL of methanol/DI H
2
O (5:95, v/v)
Elute Cefoperazone
6mL of methanol
Analysis
Mobile phase: 0.005 M tetrabutyl ammonium phosphate
(pH 3.63)/ACN (70:30, v/v)
Flow: 1mL/minute
Temperature: 30°C
Detection: UV 254nm
Recommended HPLC Column
Part Number
Hypersil GOLD aQ 5µm, 250 x 4.6mm
25305-254630
Clenbuterol in Solution
Using 60mg 3mL HyperSep Retain-CX Extraction Column
(Part Number: 60107-303)
Sample Preparation
Prepare a 10ppm solution of clenbuterol in 20mM
ammonium acetate
Condition Retain-CX Extraction Column
2mL of methanol
2mL of DI H
2
O
2mL of 30mM HCl
Apply Sample
Load 1mL samples
Wash Column
1mL of methanol
1mL of DI water
Elute Clenbuterol
1mL of methanol with 4% ammonia
Analysis
Mobile phase:
methanol:phosphoricacid (40:60, v/v)
Flow: 1mL/minute
Temperature: 30°C
Detection: UV 244nm
Recommended HPLC Column
Part Number
Hypersil GOLD aQ 5µm, 250 x 4.6mm
25305-254630
Pharmaceutical/Biotech
Applications