5
Thermo Scienti c Poster Note
•
PN ASMS13_M003_TOlney_E 07/13S
tographic response for testosterone
ernal standard (292.4
97.2)
atographic response for the internal
d the product ion mode (292.4
se for the full scan internal standard
panel. In the lower panels the
oduct Ion Mode mass spectrum are
o 2000 pg/mL range are shown in
ll outside of the predetermined
w, they track the internal standard
only two cases are the values
ations were not large enough to
tem.
Conclusion
A research method has
mass spectrometer in re
This method can be use
need for manual review.
system evaluation and/o
The method is construct
particularly are the limits
RE 4. Response curve for
sterone using standard method
SV)
fined by <15% RDS, is unchanged at
SV methods.
r IS. LOD is approximately at the
ethods.
drifts, the observed response i
table 3. This can help to elucid
Figure 8 shows a series of inje
range. The induced drift repre
associated change in peakwidt
analyte and internal standard r
The output of these measurem
and evaluation with respect to
software. Responses can be li
snapshot of the instrument sta
manual review; d) automaticall
rerun of the sample; f) triggeri
triggering a series of self-diag
the extreme, the response ma
organization that a failure has
Method Complexity
The method is constructed to
compromise, while taking a po
and product ions. To this end, l
that can be used at any given t
In general two ISV ions with fo
the method as it is currently im
chromatographic peaks or mu
Error Conditions
To demonstrate the utility of the method, a series of failures were induced in the mass
spectrometer to show the ability of the method to detect these situations. Figure 7
shows a series of injections representing Q1 mass drift across the peak. The induced
drift represents the Q1 mass position moving from 293.7 to 292.5 Da. As the mass
Measurements
esponse
% St Dev
2113
12.8%
3994
5.8%
19329
1.7%
197403
1.2%
2117342
1.2%
V Measurements
esponse
% St Dev
1922
9.7%
3824
5.6%
19301
1.1%
188281
1.3%
2029272
0.8%
RE 6. Testosterone 40 pg on
mn. Chromatograms shown from
to bottom: Testosterone d3 Q1
Scan, Product Ion Scan; Mass
ctra: Testosterone d3 Q1 Full
n, Product Ion Scan
TABLE 3. Effect on Testosterone and Internal Standard for simulated Q1 mass
drift.
TABLE 2. Testosterone response corrected for IS. Expected values and
tolerance limits are shown at the bottom.
TABLE 4. Effect on Testoster
resolution and mass drift.
Conc
fg/uL
Analyte Internal Std Resp/IS
M/Z
Width
Area
M/Z
Width
Area
40
2948 732910
2396
292.51
0.75 231442
97.23
0.68
13568
40
2492 703486
2050
292.49
0.81 221995
97.22
0.62
14817
40
2775 721359
2270
292.49
0.78 229631
97.22
0.71
15039
40
2432 637542
2247
292.47
0.82 212293
97.21
0.73
13212
40
1731 617986
1517
292.48
0.84 202647
97.23
0.76
13327
40
2425 647537
2196
292.48
0.81 215050
97.20
0.70
11670
80
4480 737877
3871
292.47
0.79 242640
97.21
0.71
14245
80
4513 785476
3637
292.48
0.73 271143
97.21
0.71
16265
80
4246 649547
4207
292.49
0.81 210047
97.22
0.67
13511
80
4036 660396
3900
292.48
0.73 198064
97.23
0.69
13579
80
4896 748874
4207
292.49
0.76 252640
97.21
0.72
17258
80
4401 682479
4143
292.48
0.76 218588
97.22
0.66
13602
400
21176 749495
19843
292.50
0.86 254163
97.24
0.75
16117
400
20982 767396
19186
292.48
0.84 230583
97.20
0.74
14974
400
15863 571678
19479
292.48
0.83 193470
97.21
0.69
9932
400
16293 587573
19465
292.47
0.75 203190
97.23
0.77
12087
400
13233 491762
18875
292.48
0.83 168075
97.20
0.63
9974
400
13344 489467
19129
292.48
0.78 163832
97.25
0.73
9507
4000 197336 702203 201837
292.49
0.79 237080
97.22
0.73
14948
4000 200435 731429 196803
292.49
0.69 239721
97.21
0.65
14157
4000 162271 597776 194950
292.48
0.80 195292
97.22
0.68
12073
4000 159666 579981 197713
292.47
0.75 185614
97.22
0.77
11653
4000 186138 677821 197221
292.49
0.82 227182
97.22
0.72
14333
4000 169513 621454 195893
292.49
0.76 205373
97.20
0.72
12130
40000 1628083 544745 2151369
292.46
0.83 190210
97.20
0.78
11908
40000 1484947 500983 2133628
292.45
0.88 171022
97.20
0.74
11208
40000 1522016 515818 2123993
292.45
0.88 169641
97.22
0.72
11265
40000 1226096 416741 2117816
292.46
0.85 137600
97.20
0.74
7165
40000 1279791 443751 2076001
292.45
0.90 144843
97.19
0.67
9442
40000 1113194 381350 2101243
292.46
0.85 121133
97.19
0.67
8480
Expect
600000
292.4
0.8
200000
97.2
0.7
12500
Error
150000
0.1
0.1
50000
0.1
0.1
3125
Min
450000
292.3
0.7
150000
97.1
0.6
9375
Max
750000
292.5
0.9
250000
97.3
0.8
15625
ISV Table for Demonstation
Response
Parent
Product
Conc
fg/uL Analyte nternal Std Resp
400 129983 4524398
400 81433 2660164
400 62903 2114164
400 71324 2315379
400 37355 1268617
400 25779 813788
400 15637 550496
400 7182 259348
400 2169 77429
400
572 19136
400
200
6462
400 ND ND
400 ND ND
ISV Table
Response
Figure 7. Simulated Q1 mass drift.
Conc
fg/uL Analyte Internal Std M/Z Width Area
M/Z Width Area
400 ND ND
ND
ND ND ND ND
ND
400 3201 101823 293.68
0.78 28377 97.21
0.71 1768
400 15765 568519 292.47
0.75 203190 97.23
0.77 11695
400 18019 568924 293.46
0.78 178890 97.23
0.77 12729
400 21541 669797 292.39
0.81 214418 97.24
0.72 15903
400 7010 221336 291.90
1.44 69596 97.24
0.73 3798
400
502 15619 292.51
1.42 5000 ND ND
ND
ISV Table for Demonstation of Q1Mass Drift
Response
Parent
Product
Figure 8. Simulated Q3 resol
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FOR RESEARCH USE ONLY. Not for use in diag
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