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6 Development and Validation of Methods for Chemotherapy Drugs on the New Prelude SPLC LC-MS/MS System
Conclusion
 Chemotherapeutic research methods were validated on the new Prelude SPLC
with method durations of 4 minutes or less.
 The Prelude SPLC system has lower void volumes than conventional HPLC
systems, resulting in sample run times that are 20-30% shorter. The reduced run
time also results in reduced cost due to lower consumption of mobile phases.
 The Prelude SPLC system uses a single syringe fill per sample, which removes
the need for pulse dampeners, reduces the mechanical wear and tear on the
pumps, does not need proportioning valves, and removes the need for active
check valves. The result: far less maintenance is required, further reducing cost
and down time.
References
1. Lambert, W.E., et al. Anal Chem. 2005, 77, 4677-4683.
2. Haouala, A. et al. J. Chrom. B. 2009, 877, 1982-1996.
3. Panderi, I. J. Chrom. B. 2009, 877, 3850-3856.
Acknowledgements
I would like to thank everyone in the Prelude research and development team for
providing support and troubleshooting in allowing this work to be accomplished.
For research use only. Not for use in diagnostic procedures.
All trademarks are the property of Thermo Fisher Scientific and its subsidiaries.
5 1.0 1.5 2.0 2.5 3.0
Methotrexate
Time (min)
LOQ for Each Compound
S
Prelude SPLC System & TSQ Endura™ MS
Cross-validation of chemotherapeutics will be repeated on the Prelude SPLC system
coupled to the new Thermo Scientific™ TSQ Endura™ triple-stage quadrupole mass
spectrometry platform.
have been done previously with liquid-
inutes)1-3. Off-line techniques such as
ly from both a material and waste
ers the sample preparation duration
mple handling. Utilizing the new
r 4 minutes. The shorter method time
sed further by the multiplexing
r run time also dramatically reduced
ns operating cost.
d to reduce instrument maintenance,
LC/MS/MS applications that require
e SPLC system utilizes syringe pumps
uired for each sample analysis with a
reduces the wear and tear on pump
piston reciprocating pumps can move
le run. The majority of maintenance
wear of the seals and check valves;
itional HPLC pumps. In addition, the
for pulse dampers. The result is that
olumes, making rapid changes in
of the steps in a method to occur are
lvent costs for equivalent methods
for Docetaxel on a Prelude
nal HPLC System
200 250 300 350
Column
Clean-up
olumn
lean-up
Equilbration
Equilbration
(sec)
Prelude SPLC System & TSQ Vantage MS