Page 219 - CRFTApplication Compendium_090613

5 Thermo Scienti c Poster Note • PN63783_E 03/13S
Conclusion
We achieved our goal of a 0.5-ng/mL LO
MDPV, mephedrone and methylone, as w
urine. Naphyrone, which shows greater v
in a qualitative manner. Deuterated intern
quantitation of these compounds.
For Forensic Toxicology use only.
ng Thermo Scientific™
blank human urine with analytes.
ilar manner at low (LQC), middle
iability and robustness were
level along with a calibration curve
ee different days. Matrix effects
te at 10 ng/mL, and internal
e of a sample prepared in water.
All trademarks are the property of Thermo Fisher Scie
This information is not intended to encourage use of th
intellectual property rights of others.
FIGURE 8. Representative chromatogra
reconstructed at 5 ppm mass accuracy.
FIGURE 9. Inter-assay QC results
Although naphyrone was detected at 0.5 ng/mL, it showed more variability than the
other compounds and a greater matrix effect from lot to lot. Absolute recoveries for
naphyrone ranged from 146% to 754% while relative recoveries using MDPV-d8 as
internal standard ranged from 150% to 596%. All available internal standards were
tried, and MDPV-d8 showed the best results. A lack of a deuterated analog for
naphyrone does not allow for matrix effect corrections and negatively effects method
precision. In this assay, naphyrone should be considered qualitative.
Quantifier m/z Qualifier m/z
160.1119 145.0885
163.1306 148.1072
160.0756 190.0861
163.0943 193.1049
126.1278 135.0440
134.1779 135.0440
141.0697 211.1115
174.0911 204.1016
179.1224 209.1329
174.0912 204.1017
177.1099 207.1205
176.1069 161.0834
butylone
LQC
2.5 ng/mL
MQC
25 ng/mL
H
100
Mean 2.28 26.0 9
%Bias -8.7 4.1 -
%CV 3.7 3.8
MDPV
LQC
2.5 ng/mL
MQC
25 ng/mL
H
100
Mean 2.30 25.7 9
%Bias -8.1 2.9 -
%CV 5.8 6.3
methedrone
LQC
2.5 ng/mL
MQC
25 ng/mL
H
100
Mean 2.49 28.1 1
%Bias -0.3 12.5
%CV 4.4 6.4
naphyrone
LQC
2.5 ng/mL
MQC
25 ng/mL
H
100
Mean 2.55 22.9 1
%Bias 2.1 -8.3
%CV 22 8.5
1.0 1.2 1.4 1.6 1.8
Time
0
20
40
60
80
100
Relative Abundance
mephed methedrone
methylone
butylone
ethylone
n energies (NCE) for cathinones
eter
0
1
2
3
4
5
6
7
8
9
10
0 200 400 600 800 1000
Area Ratio
ng/mL
methylone
R 2 = 0.9967
FIGURE 7. Representative calibration curves of cathinones in urine.
0
1
2
3
4
5
6
7
8
9
10
0 200 400 600 800 1000
Area Ratio
ng/mL
butylone
R 2 = 0.9969
0
1
2
3
4
5
6
7
8
9
10
0 200 400 600 800 1000
Area Ratio
ng/mL
ethylone
R2 = 0.9926
0
1
2
3
4
5
6
7
8
9
10
0 200 400 600 800 1000
Area Ratio
ng/mL
MDPV
R 2 = 0.9930
0
2
4
6
8
10
12
0 200 400 600 800 1000
Area Ratio
ng/mL
methedrone
R2 = 0.9950
0
1
2
3
4
5
6
7
8
9
10
0 200 400 600 800 1000
Area Ratio
ng/mL
mephedrone
R2 = 0.9900
0
1
2
3
4
5
6
0 200 400 600 800 1000
Area Ratio
ng/mL
naphyrone
R2 = 0.9805 one and butylone were all linear
tive calibration curves for all
togram at 0.5 ng/mL for all
tics shown in Figure 9 demonstrate
range for the above compounds.
pounds, and those were largely
olute recoveries of all cathinones
prepared in water ranged from
% to 136%. Precision across all
rds were used.