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6
Direct Analysis using Paper-Spray Mass Spectrometry: Method Development for the Rapid Screening of Drugs of Abuse for Forensic Toxicology
wn below. Data processed in
in the mix are positively
y isotopic pattern and the
ment. Each analyte appears twice
y peak width is twice wider than
FIGURE 6. Accurate mass (3-4 ppm) MS spectra at 70,000 resolving power showing
drugs detected down to 100 ng/mL. Sample contained six drugs analyzed from DBS.
sing software.
40
60
80
100
136.1126
40
60
80
100
150.1284
40
60
80
100
244.2069
2441922
40
60
80
100
300.1603
40
60
80
100
304.1555
40
60
80
100
318.1712
00 ng/mL
amphetamine
PCP
codeine
cocaethylene
cocaine
Meth-
amphetamine
40
60
80
100
0
20
136.1055 136.1223
136.1125
40
60
80
100
0
20
150.1132
150.1282
40
60
80
100
0
20
244.2277
244.1039
.
244.2067
244.2278
40
60
80
100
0
20
300.1431
300.1791
300.1874
300.1427
300.1604
300.1661
300.1790
40
60
80
100
0
20
304.2116
304.1408
304.1552
40
60
80
100
0
20
318.1409
318.2837
318.1708
25
00 ng/mL
40
60
80
100
0
20 136.1054 136.1222
136.1337
136.1126
40
60
80
100
0
20
150.1130
150.1932
150.1495
150.1283
40
60
80
100
0
20
244.1915
244.2470
244.2070
244.2281
244.1922
40
60
80
100
0
20
300.2024
300.1431
300.1793
300.1600
40
60
80
100
0
20
304.2130
304.1078
304.1760
304.1555
40
60
80
100
0
20
318.1403 318.2648
318.0685
318.1711
10
00 ng/mL
40
60
80
100
0
20
136.1055
136.1224
136.1338
136.1055
136.1127
40
60
80
100
0
20
150.1132
150.1367
150.1212
150.1284
150.0896
40
60
80
100
0
20
244.2473
244.1553
244.2282
40
60
80
100
0
20
300.2030
300.1432
300.1796
300.1668
40
60
80
100
0
20
304.2121
304.1082
304.1706
304.1556 304.2132
40
60
80
100
0
20
318.1408
318.2839
318.0692
318.1407
318.1923
3180691
5
00 ng/mL
Isotopic pattern match
Simulation
Experimental
136.10
m/z
0
20
136.1339
136.1224
150.10
150.15 150.20
m/z
0
20
244.1
244.2
244.3
m/z
0
20
244.2070
244.1919
244.1554
244.2469
300.15
300.20
m/z
0
20
300.2033
304.1
304.2
m/z
0
20
304.1082
318.0
318.2
m/z
0
20
.
318.2286
318.3018
1
Conclusion
• We have shown an easy to use technique (no sample preparation, no chromatography)
hrough an ‘All Ion
onfirmation in the screening of
from DBS. Examples of two
that shows extraordinary potential for screening drugs of abuse in forensic toxicology.
• The paper sample cassette allows for direct sample deposition, safe sample handling
and storage after drying. Once dry, the sample is stable for convenient shipping.
ore than one drug are shown.
76
Amphetamine,
80
90
100
119.0860
• Quantitation of drug out of dry blood spot sample is demonstrated with full MS
experiment in the Exactive Plus mass spectrometer. Analyte normalized by labeled
internal standard.
0.00-32 ...
183.0998
FTMS+p ESIFull ms2 1000.00@hcd43.00 [50.00-32 ...
methamphetamine,
m/z
119.0855
20
30
40
50
60
70
119.0194
1189722
• High resolution and accurate mass are crucial techniques for analyzing complex
samples by MS and nicely complement the paper spray technique in the screening of
drugs from dried blood spots.
119.0
119.2
m/z
0
10
.
• Data collected in this screening application allows for retrospective analysis
• Accurate mass fragments from an ‘All Ion Fragmentation’ experiment (acquired in the
same method as full MS) and isotopic pattern matching are required to confirm drugs
183
626
identified by accurate mass.
• As the electrospray signal from paper spray lasts 30 seconds and longer, MS and AIF
experiments can both be collected at ultrahigh resolution (140k resolving power).
FTMS+p ESIFull ms2 1000.00@hcd43.00 [50.00-32 ...
70
80
90
100
91.0548
• The paper spray technique coupled with automated data processing using TraceFinder
3.0 software provides a complete solution for drug screening in forensic toxicology.
20
30
40
50
60
90.9774
For forensic toxicology use only.
All trademarks are the property of Thermo Fisher Scientific and its subsidiaries.
This information is not intended to encourage use of these products in any manners that might infringe the
intellectual property rights of others.
90
91
92
m/z
0
10
92.0579
90.0922
91.6932
90.6349
91.2428
