Issue 61

Tip of the Month

New Thermo Scientific FAIMS Tutorial DVD Now Available

The most recent high-Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) tutorial is now available. This new installation tutorial has been added to the compound optimization instructions. Now you can learn how to disassemble the electrodes and impress your social network with your intimate knowledge of ion mobility mass spectrometry. FAIMS is still a cool way to clean up your chromatograms and offer a leading special capability over your competitors. Ask your sales specialist for the DVD with all three tutorials. 

Thermo Fisher Scientific Launches New Cutting-Edge Mass Spectrometry Instruments at ASMS 2011

Redefining Performance and Increasing Productivity for a Range of Applications

Three breakthrough instruments were introduced that take mass spectrometry (MS) analysis to new levels of performance in resolution, speed, sensitivity and accuracy. The new instruments were unveiled at the 59th Annual ASMS Conference on Mass Spectrometry and Allied Topics, being held June 5-9 at the Colorado Convention Center.

- Thermo Scientific Velos Pro: An ion trap with unsurpassed speed and sensitivity for MSn molecular structural elucidation and the ideal front end for Orbitrap(R) hybrid instruments.
- Thermo Scientific Orbitrap Elite: This new high-field Orbitrap instrument will revolutionize proteomics research by increasing the speed and resolution of current Orbitrap technology fourfold - a quantum leap in performance.
- Thermo Scientific Q Exactive Orbitrap: The "Quanfirmation" instrument offers the ability to identify, quantify and confirm from a single experiment across a growing number of applications, including drug metabolism and pesticide analysis.
- Thermo Scientific TSQ Quantum XLS Ultra: The new triple quadrupole GC-MS/MS system is designed to offer best-in-class selectivity, analytical performance and productivity for a wide range of GC-MS applications, including environmental, food safety, forensics, metabolomics and pharmaceutical.

More 

Proteomics

On-the-Fly Retention Time Shift Correction for Multiple Targeted Peptide Quantification by LC-MS/MS

Selected reaction monitoring (SRM) using triple quadrupole mass spectrometers is a powerful technique for targeted protein quantification due to its high sensitivity and selectivity. To satisfy growing demand for verifying large numbers of putative targets found during the discovery phase, the instrument needs to be capable of quantifying hundreds of peptides, with confirmation of their identities, in a single LC-MS run. To address this challenge, a new intelligent SRM (iSRM) technology has been incorporated into the Thermo Scientific TSQ family of triple quadrupole mass spectrometers. This technology enables processing of over 15,000 SRM transitions for simultaneous quantification and identity confirmation of up to 1,000 peptides in a single LC-MS run.

» Download the Complete Application Note 503:
On-the-Fly Retention Time Shift Correction for Multiple Targeted Peptide Quantification
[PDF 676 kB]

Drug Metabolism

High-Resolution LC-MS Analysis of Compounds for Early Drug Discovery: Quantitative and Qualitative Screening in One Experiment

Full scan, ultra-high resolution and accurate mass liquid chromatography-mass spectrometry (LC-MS) instruments allow for rapid analyses of drug discovery samples without the need for specific MS methods for target compounds. With the use of sophisticated algorithms, Thermo Scientific MetQuest software automatically processes the data obtained from these instruments to identify compounds of interest at mass-to-charge values that have not necessarily been pre-defined, while allowing the re-interrogation of the same data set for the identity and relative quantities of metabolites in both in vitro and in vivo time course studies.

» Register to Download the Complete Application Note 501:
High-Resolution LC-MS Analysis of Compounds for Early Drug Discovery: Quantitative and Qualitative Screening in One Experiment
[PDF 1.37 MB]

Full Scan Data Acquisition for Rapid Quantitative and Qualitative Analysis Using the Exactive Benchtop LC-MS High Resolution Mass Spectrometer

Current approaches to discovery stage drug metabolism studies have focused on the use of targeted analysis-based approaches, which necessitate the optimization of many parameters while detecting only the specified compound. This application note describes the use of high resolution, full scan MS, and Higher Energy Collision Cell Dissociation (HCD) data obtained by the Thermo Scientific Exactive LC/MS system for the quantitation and qualitative analysis of a tolbutamide in vitro rat hepatocyte incubation. The ability to do full scan acquisition for quantitation eliminates the need for compound optimization, while enabling the detection of metabolites and other non-drug related endogenous components.

» Register to Download the Complete Application Note 504:
Full Scan Data Acquisition for Rapid Quantitative and Qualitative Analysis Using the Exactive Benchtop LC-MS High Resolution Mass Spectrometer
[PDF 663 kB]

Analytical Tools & Resources for Drug Metabolism - Learn More Today!

Thermo Scientific brings industry-leading mass spectrometry systems & application-based intuitive software to you that provide sensitive and reliable quantitative and qualitative analysis of complex samples in both research and routine applications. Our workflow tools address the following applications to empower & accelerate your drug discovery process:

- Automated Quan/Qual Metabolic Screening workflow solutions provide early pharmaceutical discovery with rapid stability and early metabolic soft spot data.
- Definitive Biotransformation Studies workflow solutions enable you to overcome even your most difficult metabolite identification & structural elucidation challenges.
- Tissue Imaging workflow tools offer a complementary approach to the critical question of drug and metabolite localization within tissue and its relation to PK/PD (pharmacokinetics/pharmacodynamics) & TK (toxicokinetics).
- Toxicology Workflow Tools accelerate detection of potential reactive species early in the drug discovery process.

More 

Metabolomics

Accurate and Sensitive All-Ions Quantitation Using Ultra-High Resolution LC/MS

Using ultra-high resolution and accurate mass data to obtain high-quality quantitative data for metabolomic studies is an effective alternative to performing selective reaction monitoring (SRM) techniques using triple quadrupole mass spectrometry. Ultra-high resolution liquid chromatography-mass spectrometry (LC-MS) provides sensitive, high-quality, quantitative data and offers a simple, relatively unbiased, and highly quantitative assessment of metabolomics samples, negating the need for multiple mass spectrometer platforms.

» Register to Download the Complete Application Note 476:
Accurate and Sensitive All-Ions Quantitation Using Ultra-High Resolution LC/MS [PDF 503 kB]

Clinical Research and Forensic Toxicology

Quantitative Analysis of Immunosuppressant Drugs in Whole Blood Using High Throughput LC-MS/MS

Learn about a 1-minute quantitative method for research laboratories to simultaneously analyze tacrolimus, sirolimus, everolimus and cyclosporin A in whole blood.

» Register to Download the Complete Application Note 487b: Quantitative Analysis of Immunosuppressant Drugs in Whole Blood Using High Throughput LC-MS/MS [PDF 197 kB]

Food Safety

Thermo Scientific B-agonist Application Kit

One Source Total Solution Method Development - The B-agonist Application Kit helps the researcher to streamline and accelerate the food sample preparation, handling and analysis process by eliminating the need to develop an analytical method from scratch. By using the range of products provided in the kit and following its ready-made application protocol, the scientist can more easily determine B-agonist residues in food.

» Download the Product Flyer:
Thermo Scientific B-agonist Application Kit [PDF 460 kB]

Environmental

Analysis of Basic and Acidic Pharmaceutical Products in Drinking Water Using Online Sample Preparation and LC-MS/MS

Learn how the team at Bergisches Wasser- und Umweltlaborder BTV-GmbH reduced time spent on sample preparation by 95% by implementing online sample preparation.

» Register to Download the Complete Application Note 494: Analysis of Basic and Acidic Pharmaceutical Products in Drinking Water Using Online Sample Preparation and LC-MS/MS [PDF 379 kB]

Sample Introduction

Thermo Scientific Transcend System - Increase the Efficiency of Your Mass Spectrometry Workflow

The Thermo Scientific Transcend system combines the power of automated online sample preparation and multiplexing to increase the efficiency of your mass spectrometry workflow. The Transcend^TM system is optimized to increase productivity, enhance efficiency and boost throughput to your mass spectrometer - without compromising data quality or sensitivity.

»;Download the Product Brochure:
Thermo Scientific Transcend System [PDF 1.75 MB] 

;

Chromatography Columns and Consumables

Thermo Scientific Accucore HPLC Columns - Ultimate Solid Core Performance to Maximize Your Investment

Accucore^TM HPLC columns are based on Core Enhanced Technology - a unique combination of rugged and reliable solid core particles, tight control of particle diameter, advanced bonding technology and automated packing processes. Available in six different phases, Accucore HPLC columns offer long lifetimes, fast separations and superb resolution all at lower pressures than sub-2 µm columns.

» Register to Download the Free Accucore Overview Poster at www.thermoscientific.com/accucore 

The 2010 Chromatography Columns and Consumables Catalog Now Optimized for Viewing on the Apple® iPad®

This comprehensive catalog contains a robust applications search tool, as well as access to method development guides, application notebooks and other technical information to guide you through any challenge on any instrument. You will also find valuable information on the following topics:

- An extensive range of HPLC, GC and SPE products
- Reagents and Standards
- Vials and closures for any autosampler

» View the iPad-Optimized Version Online

» View the Interactive Online Version

Trap Talk

Thermo Scientific Velos Pro Dual-Pressure Linear Ion Trap LC-MSⁿ

Accelerating Innovation

The Velos Pro^TM LC-MSⁿ ion trap is the premier system for qualitative and quantitative analysis of complex proteomics and bioanalytical samples.

- Novel detection system provides up to 6 orders of linear quantitation
- Trap-HCD fragmentation increases flexibility for structural elucidation
- Generation II ion optics improves robustness and reduces downtime

» Learn More and Request Product Literature

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Additional Information

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• Tip of the Month
• ASMS Product Launches
• Proteomics
• Drug Metabolism
• Metabolomics
• Clinical Research and Forensic Toxicology
• Food Safety
• Environmental
• Sample Introduction
• Chromatography Columns and Consumables
• Trap Talk