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Conclusion
We have developed and fully validated a simple, fast and
sensitive LC-APCI-MS/MS method for measurement of
E1 and E2 in serum/plasma without derivatization. The
LLOQ for E1 and E2 are 3.5 and 4.4 pg/mL, respectively.
The method was linear between 3.5 and 1019.3 pg/mL for
E1, and 4.4 and 1032.5 pg/mL for E2. No ion suppression
or carryover was observed. In addition, for clinical
research laboratories, this method offers high precision
and recovery.
For Research Use Only. Not for use in diagnostic procedures.
Retention Time (min)
2.1
2.4
2.7
3.0
3.3
3.6
3.9
0
379
E1:
269 145
16.9 pg/mL
E2:
271 183
25.1 pg/mL
SRM:
SRM:
Figure 7: SRM chromatograms of E1 and E2 in human plasma sample 2 (female)