Mass Spectrometry
Thermo Scientific TSQ Quantum Ultra triple stage quadrupole mass spectrometer
Ion Source & Polarity: APCI, positive ion mode
Discharge Current:
4.0 µA
Vaporizer Temperature: 325 °C
Sheath Gas:
60 units
Ion Sweep Gas:
0 units
Auxillary Gas:
10 units
Capillary Temperature: 275 °C
Collision Gas Pressure: 1.5 mTorr
The selective reaction monitoring (SRM) transitions used
are presented in Table 2.
Scan Collision Tube
Analyte
Parent Product Time Energy Lens
Clozapine
327.20 192 25 ms
60
47
270 25 ms
21
47
Norclozapine 313.20 164 25 ms
67
113
192 25 ms
41
113
Table 2: SRM transitions monitored in the experiment
Figure 2: Extracted ion chromatogram of the plasma blank